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过程工程学报 ›› 2017, Vol. 17 ›› Issue (6): 1257-1264.DOI: 10.12034/j.issn.1009-606X.217180

• 过程与工艺 • 上一篇    下一篇

利用新型膜乳化器制备粒径均一的PLGA微球

赵 培1， 李 艳1， 王建梅1， 王雪莹1， 许 敏1， 王立秋1,2*   

  1. 1. 山东省科学院能源研究所，山东 济南 250014；2. 香港大学机械工程系，香港
  • 收稿日期:2017-03-17 修回日期:2017-04-25 出版日期:2017-12-20 发布日期:2017-12-05
  • 通讯作者: 王立秋 lqwang@hku.hk
  • 基金资助:
    体外水凝胶肝血管模型构建及其用于载药微球释药行为研究;体外研究病理条件下流体剪切力对药物释放的影响;气动微流控芯片三维微血管构建用于栓塞微球栓塞行为研究; 基于气动微流控芯片的三维圆截面微血管构建;Engineering Embolic Microparticles with Uniform Shape and Size;Microfluidic Fabrication of Thermally-Smart Nanoparticle Colloidosomes with Single or Multiple Compartments;Chemoembolization Microparticles: Microfluidic Fabrication and Characterization;Engineering Water and Oil with Ultra-high Thermal Conductivity

Preparation of Uniform-sized PLGA Microspheres Based on Novel Membrane Emulsification

Pei ZHAO1, Yan LI1, Jianmei WANG1, Xueying WANG1, Min XU1, Liqiu WANG1,2*   

  1. 1. Institute of Energy Research, Shandong Academy of Sciences, Jinan, Shandong 250014, China; 2. Department of Mechanical Engineering, University of Hongkong, Hongkong, China
  • Received:2017-03-17 Revised:2017-04-25 Online:2017-12-20 Published:2017-12-05
  • Contact: Liqiu QiuWang lqwang@hku.hk

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摘要： 用恒流泵替代气瓶给压装置得到新型膜乳化器，以改善膜乳化法制备微球的工艺稳定性；以聚乳酸?羟基乙酸共聚物(PLGA)为膜材，利用水包油(O/W)乳化法制备PLGA微球，研究了新型膜乳化器与传统膜乳化器对微球形貌、粒径及粒径分布的影响，考察了搅拌速度、稳定剂浓度、PLGA浓度及分散相流速对微球形貌、粒径及其分布的影响. 结果表明，在微孔膜孔径2 ?m、搅拌速度300 r/min、稳定剂浓度1.0%(?)及PLGA浓度60 mg/mL、分散相流速20 mL/h的条件下，可制得表面光滑、粒径均一(粒径分布系数Rspan<0.50)的PLGA微球，各批产品的粒径相对标准偏差为1.99%，产品批次重复性良好.

关键词: 膜乳化, 压力控制, PLGA微球, 粒径均一, 重复性

Abstract: A novel pressure control method (constant-flow pump) was introduced to conventional membrane emulsification, for precisely controlling the transmembrane pressure of the dispersed phase, named novel membrane emulsification. Poly(lactic-co-glycolic acid) (PLGA) microspheres was prepared by oil-in-water emulsion method respectively, based on conventional membrane emulsification and novel membrane emulsification. Compared with conventional membrane emulsification, this novel membrane emulsification could remarkably improve the size distribution of PLGA microspheres. The processing conditions, stirring rate, the stabilizer concentration, the PLGA concentration and the dispersed phase flow rate, were optimized to fabricate PLGA microspheres with controllable sizes and narrow size distribution. The results showed that uniform-sized PLGA microspheres (size distribution coefficient Rspan<0.50) were successfully fabricated using 2 ?m microporous membraneunder the conditions of stirring speed about 300 r/min, the stabilizer concentration of 1.0%(?), the PLGA concentration of 60 mg/mL and the dispersed phase flow rate of 20 mL/h. The relative standard deviation was 1.99%, and the product repeatability was good.

Key words: membrane emulsification, control of pressure, PLGA microsphere, narrow size distribution, repeatability