BAF工艺预处理含微囊藻毒素微污染水源的微生物特征

doi:10.12034/j.issn.1009-606X.217354

过程工程学报 ›› 2018, Vol. 18 ›› Issue (4): 866-871.DOI: 10.12034/j.issn.1009-606X.217354

BAF工艺预处理含微囊藻毒素微污染水源的微生物特征

张文艺1*，王逸超1，蔡庆庆1,2

1. 常州大学环境与安全工程学院，江苏常州 213164；
2. 江苏龙环环境科技有限公司，江苏常州 213127

收稿日期:2017-10-10 修回日期:2017-12-19 出版日期:2018-08-22 发布日期:2018-08-15
通讯作者: 张文艺 zhangwenyi888@sina.com
基金资助:
国家自然科学基金青年项目;“十三五”国家水体污染控制与治理科技重大专项

Microbial characterization of micro polluted source water containing microcystin pretreatment with biological aerated filter

Wenyi ZHANG1*, Yichao WANG1, Qingqing CAI1,2

1. School of Environmental & Safety Engineering, Changzhou University, Changzhou, Jiangsu 213164, China
2. Jiangsu Longhuan Environmental Science Co., Ltd., Changzhou, Jiangsu 213127, China

Received:2017-10-10 Revised:2017-12-19 Online:2018-08-22 Published:2018-08-15

摘要/Abstract

摘要： 以曝气生物滤池载体表面的生物膜为研究对象，通过镜检、磷脂脂肪酸(PLFA)测定、高通量454测序等手段研究了生物膜降解含氮有机物和微囊藻毒素-亮氨酸(MC-LR)的微生物特征. 结果表明，低碳源的微污染水源中水生物膜的微生物群落极为丰富，形成了由好氧细菌组成的微生物群落，运行前2周有6大门类17大种属，3?4周增加到14大门类43大种属，还有线虫、草履虫、水蚤等原生动物；优势菌属有球衣菌属Sphaerotilus (2.41%?24.58%)、气单胞属Aeromonas (4.16%?12.59%)、黄杆菌属Cloacibacterium (1.85%?12.39%)、水杆菌属Aquabacterium (1.53%?6.76%)、噬氢菌属Hydrogenophaga (1.12%?5.9%)、嗜甲基菌属Methyloversatilis (0.53%?1.52%)、红杆菌属Rhodobacter (0.09%?1.39%)等. 此外，投加0.16% T1菌剂芽孢杆菌后其含量增至1.97%，表明投加T1降解菌后芽孢杆菌迅速生长并成为优势种群，可强化降解MC-LR.

关键词: 水源水, 曝气生物滤池, 高通量454, 微生物

Abstract: The microbial characteristics of biodegradation of organic compounds containing nitrogen and microcystin LR (MC-LR) was researched with the biofilm of biological aerated filter (BAF) reactor surface as the research object, by the means of microscopic examination, phospholipid fatty acid (PLFA), high-throughput sequencing and others. The results showed that the microbial community on the biofilm of BAF was extremely rich in the micro-polluted source water of low carbon source, formed the microbial community consists of aerobic bacteria, sulfate reducing bacteria and other bacteria. In the first 2 weeks, the microflora on the biofilm had 17 major genera and 6 species. In the 3 to 4 weeks, the microflora will increase to 43 major genera and 14 species. There also were protists such as nematodes, paramecium and daphnia. The dominant species were Sphaerotilus (2.41%?24.58%), Aeromonas (4.16%?12.59%), Cloacibacterium (1.85%?12.39%), Aquabacterium (1.53%?6.76%), Hydrogenophaga (1.12%?5.9%), Methyloversatilis (0.53%?1.52%), Rhodobacter (0.09%?1.39%), etc. In addition, the content of bacillus increased from the initial 0.16% to 1.97% after the addition of T1 degrading bacteria, which showed that bacillus grew rapidly and became dominant population after adding T1 degrading bacteria, thus enhancing the degradation of MC-LR.

Key words: Drinking water sources, Biological aerated filter, High-throughput sequencing 454

张文艺王逸超蔡庆庆. BAF工艺预处理含微囊藻毒素微污染水源的微生物特征[J]. 过程工程学报, 2018, 18(4): 866-871.

Wenyi ZHANG Yichao WANG Qingqing CAI. Microbial characterization of micro polluted source water containing microcystin pretreatment with biological aerated filter[J]. Chin. J. Process Eng., 2018, 18(4): 866-871.

参考文献

[1]叶少帆, 王志伟, 吴志超.微污染水源水处理技术研究进展和对策分析[J].水处理技术, 2010, 36(6):22-28
[2]窦娜莎.曝气生物滤池处理城市污水的效能与微生物特性研究[D]. 中国海洋大学, 2013.
[3]严子春, 何强, 龙腾锐, 等.折流曝气生物滤池的微生物特性研究[J].中国给水排水, 2011, 27(13):73-76
[4]张文艺, 夏绍凤, 董清玉, 等.上向流曝气生物滤池中有机氮沿程转化规律与生物特性研究[J].农业工程学报, 2008, 24(6):234-238
[5]胡林潮, 周新程, 邓文, 等.潜流式人工湿地消纳城市污水厂尾水微生物特性及机制[J].土木建筑与环境工程, 2016, 38(6):134-140
[6]White D C, Flemming C A, Leung K T, et al.In situ microbial ecology for quantitative appraisal,monitoring,and risk assessment of pollution remediation in soils,the subsurface,the rhizosphere and in biofilms[J].Journal of Microbiological Methods, 1998, 32(2):93-105
[7]张燕, 周巧红, 张丽萍, 等.冬季湿地植物根际微生物群落结构多样性分析[J].环境科学与技术, 2013, 36(11):108-111
[8]Whittaker P, Day J B, Curtis S K, et al.Evaluating the use of fatty acid profiles to identify Francisella tularensis[J].Journal of Aoac International, 2007, 90(2):465-469
[9]陶敏, 贺锋, 徐洪, 等.氧调控下人工湿地微生物群落结构变化研究[J].农业环境科学学报, 2012, 31(6):1195-1202
[10]Sakamoto K, Iijima T, Higuchi R.Use of specific phospholipid fatty acids for identifying and quantifying the external hyphae of the arbuscular mycorrhizal fungus Gigaspora rosea[Erratum: 2005 May,v.37,no.5,p.1009.][J].Soil Biology & Biochemistry, 2004, 36(11):1827-1834
[11]Kimura M, Asakawa S.Comparison of community structures of microbiota at main habitats in rice field ecosystems based on phospholipid fatty acid analysis[J].Biology and Fertility of Soils, 2006, 43(1):20-29
[12]温强, 王晓燕.北京城市湖泊沉积物中微生物磷脂的分布特征[J].北京: 首都师范大学学报自然科学版, 2012, 33(3):80-85
[13]尹勇, 冯国勇, 周静, 等.生态强化法原位净化村镇污水微生物特性[J].环境化学, 2015, 34(4):792-797
[14]Michaud L, Giudice A L, Troussellier M, et al.Phylogenetic characterization of the heterotrophic bacterial communities inhabiting a marine recirculating aquaculture system[J].Journal of Applied Microbiology, 2009, 107(6):1935-1942
[15]黄志涛, 宋协法, 李勋等.基于高通量测序的石斑鱼循环水养殖生物滤池微生物群落分析[J].农业工程学报, 2016, 32(1):242-246

BAF工艺预处理含微囊藻毒素微污染水源的微生物特征

Microbial characterization of micro polluted source water containing microcystin pretreatment with biological aerated filter

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