高固多相生物反应工程

doi:10.12034/j.issn.1009-606X.218232

过程工程学报 ›› 2018, Vol. 18 ›› Issue (5): 918-923.DOI: 10.12034/j.issn.1009-606X.218232

• 中科院过程工程所60周年特邀 • 上一篇下一篇

高固多相生物反应工程

王岚¹，刘阳^1,2，陈洪章¹?

1. 中国科学院过程工程研究所生物质炼制工程北京市重点实验室，北京 100190 2. 中国科学院大学化学工程学院，北京 100190

收稿日期:2018-06-12 修回日期:2018-07-25 出版日期:2018-10-22 发布日期:2018-10-12
通讯作者: 陈洪章 hzchen@home.ipe.ac.cn
基金资助:
中国科学院洁净能源先导科技专项;2017年度北京创新基地培育与发展专项基金

High-solid and multi-phases bio-reaction engineering

Lan WANG¹, Yang LIU^1,2, Hongzhang CHEN^1*

1. Beijing Key Laboratory of Biorefining Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China
2. School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100190, China

Received:2018-06-12 Revised:2018-07-25 Online:2018-10-22 Published:2018-10-12
Contact: CHEN Hong-zhang hzchen@home.ipe.ac.cn

摘要/Abstract

摘要： 以木质纤维素高固酶解发酵为例，高固形物及其复杂的理化性质会导致体系出现“固体效应”和“水束缚效应”，形成传质速度较低的固液气和微生物复杂多相体系，影响木质纤维素生物转化速率；同时，固形物增加及由此产生的高固酶解发酵流变学特性使以剪切力为主导的机械搅拌在高固多相体系下具有不适应性(会导致酶或微生物活性降低)，从而对搅拌方式、反应器及过程设计放大等提出新的要求. 本工作基于课题组多年的研究，提出高固多相生物反应工程的理念，从固体基质特性入手剖析影响高固多相生物反应速率的关键因素，提出了以周期法向力为动力源的过程强化方法，开发出周期蠕动高固多相生物反应系统，以期为高固多相生物反应工程研究提供理论和技术支持.

关键词: 高固多相, 生物反应工程, 固体效应, 水束缚, 流变学, 周期过程强化

Abstract: Low solid loading bio-reaction system lead to high energy and water consumption, as well as environment pollution in fermentation industries, ing the development of high-solid bio-reaction system. High-solid bio-reaction system has the advantages of low energy consumption, less used water and environmentally-friendly. Additionally, high-solid loading leads to higher concentration of substrate and products which are benefit to reduce cost and realize the economical efficiency in production. However, many difficulties have existed in high-solid bio-reaction system apart form so many advantages. Therefore, it is of great mean to get knowledge about the characteristics and problem of high-solid bio-reaction system which will help to make the most use of its advantages. Taking enzymatic hydrolysis and fermentation of lignocellulose for an example, increasing solid loading leads to ‘solid effects’ and ‘water constraint’. Those phenomenon result in the formation of complex multi-phase system consisted by solid, liquid, gas and the microorganism. Mass transfer performance in this high-solid and multi-phases system is deficient, thus affecting lignocellulose conversion performance. Additionally, the changes of rheology characteristics in high-solid and multi-phases bio-reaction system caused by increasing solid loading make it inappropriate to apply traditional mechanical agitation which is based on shear force (i.e. shear force can results in activity loss of the enzyme and microorganism) to high-solid and multi-phases bio-reaction system, putting new requirements on agitation methods, design and amplification of bioreactor and process of high-solid and bio-reaction system. Based on several-years studies, the conception of high-solid and multi-phases bio-reaction engineering was proposed. Key factors which affect the reaction performance were analyzed from the perspective of solid matrix characteristic. Intensification method based on periodical normal force is innovated and periodic peristalsis high-solid and multi-phases bio-reaction system was developed with the expectation of providing theory and technical support for studying high-solid and multi-phases bio-reaction engineering.

Key words: high-solid and multi-phases, bio-reaction engineering, solid effects, water constraint, rheology, periodic process intensification

王岚刘阳陈洪章. 高固多相生物反应工程[J]. 过程工程学报, 2018, 18(5): 918-923.

Lan WANG Yang LIU Hongzhang CHEN. High-solid and multi-phases bio-reaction engineering[J]. Chin. J. Process Eng., 2018, 18(5): 918-923.

参考文献

[1]Chen H Z, Liu Z H.Enzymatic hydrolysis of lignocellulosic biomass from low to high solids loading[J].Engineering in Life Sciences, 2017, 17(5):489-499
[2] Kristensen J B, Felby C, Jorgensen H.Yield-determining factors in high-solids enzymatic hydrolysis of lignocellulose[J].Biotechnology for Biofuels, 2009, 2:-
[3]Zhao J Y, Chen H Z.Correlation of porous structure,mass transfer and enzymatic hydrolysis of steam exploded corn stover[J].Chemical Engineering Science, 2013, 104(12m):1036-1044
[4]Zhu J, Wang G, Pan X, et al.Specific surface to evaluate the efficiencies of milling and pretreatment of wood for enzymatic saccharification[J].Chemical Engineering Science, 2009, 64(3):474-485
[5]Yang D, Parlange J Y, Walker L P.Revisiting size-exclusion chromatography for measuring structural changes in raw and pretreated mixed hardwoods and switchgrass[J].Biotechnol Bioeng, 2015, 112(3):549-559
[6]Arantes V, Saddler J N.Cellulose accessibility limits the effectiveness of minimum cellulase loading on the efficient hydrolysis of pretreated lignocellulosic substrates[J].Biotechnology for biofuels, 2011, 4(1):1-17
[7]Tanaka M, Ikesaka M, Matsuno R, et al.Effect of pore size in substrate and diffusion of enzyme on hydrolysis of cellulosic materials with cellulases[J].Biotechnology & Bioengineering, 1988, 32(5):698-706
[8]Grethlein H E.The Effect of Pore Size Distribution on the Rate of Enzymatic Hydrolysis of Cellulosic Substrates[J].Nature Biotechnology, 1985, 3(2):155-160
[9]Wang Q Q, He Z, Zhu Z, et al.Evaluations of cellulose accessibilities of lignocelluloses by solute exclusion and protein adsorption techniques[J].Biotechnology & Bioengineering, 2012, 109(2):381-389
[10] Chen, H Z.Gas Explosion Technology and Biomass Refinery[M]. Springer Netherlands, 2015.
[11]Liu Z H, Chen H Z.Biomass–Water Interaction and Its Correlations with Enzymatic Hydrolysis of Steam-Exploded Corn Stover[J].Acs Sustainable Chemistry & Engineering, 2016, 4(3):1274-1285
[12] Shao M A, Wang Q J, Huang M B.Soil Physics[M]. Beijing: High Education Press, 2006.
[13] 张玉针.生物质多孔颗粒特性及其高固酶解发酵过程的研究[D]. 中国科学院大学(中国科学院过程工程研究所), 2017.
[14] Selig M J, Thygesen L G, Felby C.Correlating the ability of lignocellulosic polymers to constrain water with the potential to inhibit cellulose saccharification[J].Biotechnology for Biofuels, 2014, 7:-
[15]Roberts K M, Lavenson D M, Tozzi E J, et al.The effects of water interactions in cellulose suspensions on mass transfer and saccharification efficiency at high solids loadings[J].Cellulose, 2011, 18(3):759-773
[16]Felby C, Thygesen L G, Kristensen J B, et al.Cellulose–water interactions during enzymatic hydrolysis as studied by time domain NMR[J].Cellulose, 2008, 15(5):703-710
[17]Roche C M, Dibble C J, Knutsen J S, et al.Particle concentration and yield stress of biomass slurries during enzymatic hydrolysis at high-solids loadings[J].Biotechnology & Bioengineering, 2009, 104(2):290-300
[18]Um B H, Hanley T R.A comparison of simple rheological parameters and simulation data for Zymomonas mobilis fermentation broths with high substrate loading in a 3-L bioreactor[J].Appl Biochem Biotechnol, 2008, 145(1-3):29-38
[19] Liu Z H, Chen H Z.Periodic peristalsis enhancing the high solids enzymatic hydrolysis performance of steam exploded corn stover biomass[J].Biomass & Bioenergy, 2016, 93:13-24
[20] Liu Z H, Chen H Z.Periodic peristalsis releasing constrained water in high solids enzymatic hydrolysis of steam exploded corn stover[J].Bioresource Technology, 2016, 205:142-152
[21] Chen H Z.Chen H Z. High-solid and Multi-phase Bioprocess Engineering: Theory and Practice [M]. Springer Singapore,2018.

高固多相生物反应工程

High-solid and multi-phases bio-reaction engineering

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics