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过程工程学报 ›› 2021, Vol. 21 ›› Issue (3): 314-322.DOI: 10.12034/j.issn.1009-606X.220088

• 生化工程 • 上一篇    下一篇

两室气体互通对光合微生物燃料电池性能的影响

朱馨彤1， 何 欢1， 朱润云2， 徐志昂1， 韩丰霞1*， 普红平1   

  1. 1. 昆明理工大学环境科学与工程学院，云南 昆明 650500 2. 云南省农业技术推广总站，云南 昆明 650000
  • 收稿日期:2020-03-16 修回日期:2020-05-22 出版日期:2021-03-22 发布日期:2021-03-23
  • 通讯作者: 韩丰霞 hanfengxia@kust.edu.cn
  • 基金资助:
    溶解性有机质对电化学-微生物耦合降解17α-乙炔基雌二醇的介导机制;光电耦合催化降解再生水中类固醇雌激素及协同灭菌机制

Effects of two-compartment gas interflow on the performance of photosynthetic microbe fuel cell

Xintong ZHU1, Huan HE1, Runyun ZHU2, Zhiang XU1, Fengxia HAN1*, Hongping PU1   

  1. 1. Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, Yunnan 650500, China 2. Yunnan Agricultural Technology Extension Station, Kunming, Yunnan 650000, China
  • Received:2020-03-16 Revised:2020-05-22 Online:2021-03-22 Published:2021-03-23

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摘要： 以斜生栅藻生长产生氧为电子受体的光合微生物燃料电池(PMFC)和外加CO2光合微生物燃料电池(AC-PMFC)联合构建成微生物碳捕获电池(MCC)。研究MCC在不同运行条件下的产电性能及影响因素。测量MCC, PMFC和AC-PMFC三种系统中的电压、溶解氧和pH。结果表明，产电压趋势与所有系统中的藻类阴极的氧浓度相关，电解液pH也能影响MFC电压的产生。三种类型的MFC中，MCC产电性能最佳，其电压和功率密度分别可达492 mV和102.3 mW/m2，最大功率密度分别比PMFC和AC-PMFC高42.33%, 54.08%。AC-PMFC由于添加了相对高浓度的CO2，抑制了微藻的生物活性和光合作用，产生的电压和功率密度最低。用SEM观察长期运行后的MCC的阴极表面藻类的形貌特征，藻生物膜与电极板表面能够生成一层高浓度的原位氧膜。电化学分析表明斜生栅藻–生物膜本身不能直接接收来自极板上的电子，无生物催化活性。但这层膜可促进O2的还原速率且可有效降低电池内阻。PCR和16S rRNA基因检测技术分析结果表明，MFC中的Chao1指数为170，而PMFC为152，MCC为145，阴极中的过饱和氧可通过管道输送到阳极并影响阳极的微生物群落。本研究结果为进一步改善藻类微生物碳捕获电池性能提供基础。

关键词: 燃料电池, 微生物碳捕获电池, 藻类, 生物能源, 微生物多样性

Abstract: Microbial carbon capture cell (MCC) was assembled with a photo biocathode where growing Scenedesmus obliquus to produce oxygen as an electron acceptor after the operation of photo-microbe fuel cell (PMFC) and an added CO2 photo-MFC (AC-PMFC). The voltage generation, dissolved oxygen and pH were measured over each day in the different systems. It was demonstrated that cell voltage produced by MFC was in line with the oxygen concentration in all systems with algae cathode. The pH of the electrolyte can also affect voltage generation. The highest voltage and power density of MCC were obtained in the three types of MFC with 492 mV and 102.3 mW/m2, respectively. Its maximum power density was higher than that of PMFC and AC-PMFC. The three systems received different concentrations of carbon dioxide for photosynthesis. The AC-PMFC achieved the lowest voltage and power density due to the excessive concentration of CO2, which could inhibit the biological activity and photosynthesis of microalgae. The scanning electron microscope (SEM) was measured to observe the morphology characteristics of algae on the cathode surface of MCC after long-term operation. A layer of in situ oxygen film with high concentration could be generated on the surface of algae biofilm and electrode plate. The electrochemical analysed demonstrated that the biofilm could not directly receive the electrons from the plate and had no biocatalytic activity. This biofilm could increase the rate of oxygen reduction, which can effectively reduce the resistance of the battery surface. Polymerase chain reaction (PCR) and 16S rRNA gene detection technology indicated that the Chao1 index in MFC was 170, while the PMFC was 152 and the MCC was 145. The oversaturated oxygen in the cathode could be transported to the anode by pipeline and affect the microbial community in the anode. This study could provide a basis for further understanding of algae-based microbial carbon-trapping cells to improve MCC performance.

Key words: fuel cell, microbial carbon capture cell, algae, bioenergy, microbial diversity